Caspr1 Overexpression Lysate (Native)

Images

 
Western Blot: CASPR Overexpression Lysate (Adult Normal) [NBL1-09337] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CASPR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Caspr1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC218019 and based on accession number NM_003632. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens contactin associated protein 1 (CNTNAP1), mRNA.
Gene
CNTNAP1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
154.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Caspr1 Overexpression Lysate (Native)

  • Caspr
  • Caspr1
  • CASPRcaspr1
  • CNTNAP
  • CNTNAP1
  • contactin associated protein 1
  • neurexin 4
  • Neurexin IV
  • neurexin-4
  • NRXN4contactin-associated protein 1
  • p190

Background

The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5414
Species: Hu
Applications: WB, Simple Western
AF5129
Species: Hu
Applications: WB
NBP1-62489
Species: Hu
Applications: WB
NBP2-49229
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF3235
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP1-42802
Species: Hu, Mu, Rt, Po, Bv, Ca, Xp, Ze
Applications: WB, ICC/IF, IP
NBP1-88182
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-48320
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-58359
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-25159
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NBP2-67704
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
H00002965-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
NBP1-31381
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-37399
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
NBP2-02627
Species: Hu, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-38645
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB100-91273
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for Caspr1 Lysate (NBL1-09337) (0)

There are no publications for Caspr1 Lysate (NBL1-09337).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Caspr1 Lysate (NBL1-09337) (0)

There are no reviews for Caspr1 Lysate (NBL1-09337). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspr1 Lysate (NBL1-09337) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Caspr1 Lysate (NBL1-09337)

Discover related pathways, diseases and genes to Caspr1 Lysate (NBL1-09337). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Caspr1 Lysate (NBL1-09337)

Discover more about diseases related to Caspr1 Lysate (NBL1-09337).
 

Pathways for Caspr1 Lysate (NBL1-09337)

View related products by pathway.

PTMs for Caspr1 Lysate (NBL1-09337)

Learn more about PTMs related to Caspr1 Lysate (NBL1-09337).

Blogs on Caspr1

There are no specific blogs for Caspr1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

RhoA Antibody
NB100-91273

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Caspr1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CNTNAP1