Caspase-8 Assay Kit (Fluorometric)

Product Discontinued

Caspase-8 Assay Kit (Fluorometric) Summary

• Description: Activation of caspases plays a central role in apoptosis. The Caspase-8 Assay Kit provides a convenient means for sensitive detection of activated caspase-8 in living cells. The assay utilizes the caspase-8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a fluorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells.
  
  Detection method: Fluorescence microscopy, fluorescence plate reader, Flow cytometry, L-1 channel (Ex/Em = 485/535 nm).Applications: Detection of activated caspases in living cells.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65614)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CASP8

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunofluorescence

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-8 Assay Kit (Fluorometric)

• AIS
• androgen receptor
• CASP8
• Caspase8
• Caspase-8
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• Mch5
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

FAQs for Caspase-8 Kit (NBP2-54862). (Showing 1 - 1 of 1 FAQ).

1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
   • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Bioinformatics

• Gene Symbol: CASP8