Caspase-8 Activity Assay Kit (Colorimetric)

Caspase-8 Activity Assay Kit (Colorimetric) [NBP2-54817] - The assay was performed on murine insulinoma cell line following Novu's protocol.

• Reactivity: Hu, Mu, Rt, Ma
• Suitable Sample Type: Cell and tissue lysates

Caspase-8 Activity Assay Kit (Colorimetric) Summary

• Description: Simple and convenient colorimetric assay to measure Caspase-8/FLICE activity within 1-2 hrs.
  
  Detection method: Absorbance (400 or 405 nm)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab39700)
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CASP8

Applications/Dilutions

Reviewed Applications	Read 1 Review rated 5 using NBP2-54817 in the following application:
Publications	Read Publications using NBP2-54817.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-8 Activity Assay Kit (Colorimetric)

• AIS
• androgen receptor
• CASP8
• Caspase8
• Caspase-8
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• Mch5
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Caspase-8 Kit (NBP2-54817)(2)

Publications using NBP2-54817

• Zhang R, Wu T, Zheng P et al. Thymoquinone sensitizes human hepatocarcinoma cells to TRAIL-induced apoptosis via oxidative DNA damage DNA Repair 2021-04-01 [PMID: 33990030]
• Sun C, Chesnokov V, Larson G, Itakura K Glucosamine Enhances TRAIL-Induced Apoptosis in the Prostate Cancer Cell Line DU145 Medicines (Basel) 2019-10-15 [PMID: 31618900]

Review for Caspase-8 Kit (NBP2-54817) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Francesca Pacifici 12/06/2018

Summary

• Lot: 4A11K01130

Comments

• Comments: The assay was performed on murine insulinoma cell line by following manufacturer's protocol.

FAQs for Caspase-8 Kit (NBP2-54817). (Showing 1 - 1 of 1 FAQs).

1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
   • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Bioinformatics

• Gene Symbol: CASP8