Human Caspase-8 ELISA Kit (Colorimetric)

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ELISA: Caspase-8 ELISA Kit [NBP1-83733] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, cell lysate, serum
Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human Caspase-8 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.16 - 10.0 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
8.5%
Intra-Assay
6.7%
Spike Recovery
89.5%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CASP8

Applications/Dilutions

Application Notes
The human Caspase-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-8

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Stop Solution (1M Phosphoric acid)
  2. Sample Diluent
  3. Substrate Solution (tetramethyl-benzidine)
  4. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  5. Human Caspase-8 Standard lyophilized, 20.00 ng/ml upon reconstitution
  6. Lysis Buffer 10x
  7. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  8. anti-human Caspase-8 polyclonal (rabbit) Detection Antibody
  9. Anti-rabbit-IgG-HRP
  10. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-8
  11. Adhesive Films

Alternate Names for Human Caspase-8 ELISA Kit (Colorimetric)

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Caspases are the executioners of apoptosis. These cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. Caspase-8 has been reported to have additional functions unrelated to cell death. It is required for T-cell homeostasis, proliferation, and cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

View specific protocols for Caspase-8 ELISA Kit (NBP1-83733): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 ELISA Kit (NBP1-83733). (Showing 1 - 1 of 1 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Blogs on Caspase-8.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP8
Entrez