Caspase-6 Antibody - (cleaved) - BSA Free

Western Blot: Caspase-6 Antibody - (cleaved) [NBP3-13380] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase-6 (cleaved Asp162) antibody (NBP3-13380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: Caspase-6 Antibody - (cleaved) [NBP3-13380] - Caspase-6 (cleaved Asp162) antibody detects Caspase-6 (cleaved Asp162) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat esophagus. Caspase-6 (cleaved Asp162) stained by Caspase-6 (cleaved Asp162) antibody (NBP3-13380) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Caspase-6 Antibody - (cleaved) [NBP3-13380] - Caspase-6 (cleaved Asp162) antibody detects Caspase-6 (cleaved Asp162) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Caspase-6 (cleaved Asp162) stained by Caspase-6 (cleaved Asp162) antibody (NBP3-13380) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Caspase-6 Antibody - (cleaved) - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Caspase-6 (cleaved Asp162). The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Caspase-6 Antibody - (cleaved) - BSA Free

• Apoptotic protease Mch-2
• CASP6
• CASP-6
• caspase 6, apoptosis-related cysteine peptidase
• caspase 6, apoptosis-related cysteine protease
• Caspase6
• Caspase-6
• EC 3.4.22
• Mch2
• MCH2EC 3.4.22.59

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP6