Recombinant Human Caspase-2 Protein

Product Discontinued

Recombinant Human Caspase-2 Protein Summary

• Description: Recombinant protein for Human Caspase-2
  
  

  Source:E. coli.
  Amino Acid Sequence:(P42575)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CASP2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Each Definition: One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
  Molecular Weight: two large (19 ) and two small (12 ) subunits
  Specific activity: 10,000 -13,000 units/mg
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to 1 unit per ul in PBS containing 15% glycerol.

Alternate Names for Recombinant Human Caspase-2 Protein

• caspase-2
• CASP2
• CASP-2
• caspase 2, apoptosis-related cysteine peptidase
• Caspase2
• Caspase-2
• ICH1
• ICH-1
• NEDD2
• NEDD-2
• PPP1R57

Background

Caspase-2 (also know as Ich-1, Nedd-2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis pro-caspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed active caspase-2 is a heterotetramer consisting of two large (19 kDa) and two small (12 kDa) subunits.The recombinant active human caspase-2 was expressed in E. coli. The active caspase-2 preferentially cleaves caspase-2 substrates (e.g., VDVAD-AFC or VDVAD-pNA) and is routinely tested at for its ability to enzymatically cleave these two substrates Ac-VDVAD-pNA (Cat. #1072-200) or Ac-VDVAD-AFC (1071-200).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP2