Caspase-2 Assay Kit (Fluorometric)

Product Discontinued

Caspase-2 Assay Kit (Fluorometric) Summary

• Description: Simple and convenient fluorometric assay to measure Caspase-2 activity within 1-2 hrs.
  
  Detection method: Fluorescence (Ex/Em = 400/505 nm)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: K116-100)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CASP2

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-2 Assay Kit (Fluorometric)

• caspase-2
• CASP2
• CASP-2
• caspase 2, apoptosis-related cysteine peptidase
• Caspase2
• Caspase-2
• ICH1
• ICH-1
• NEDD2
• NEDD-2
• PPP1R57

Background

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (WLmax = 400 nm); upon cleavage of the substrate by caspase-2, free AFC emits a yellow-green fluorescence (WLmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP2