Caspase-12 Antibody

Images

 
Western Blot: Caspase-12 Antibody [NBP1-77290] - Analysis of caspase-12 in (A) human and (B) mouse spleen tissue lysates with caspase-12 antibody at 1 ug/mL.
Immunohistochemistry: Caspase-12 Antibody [NBP1-77290] - Caspase-12 in human heart tissue with caspase-12 antibody at 10 ug/mL.
Western Blot: Caspase-12 Antibody [NBP1-77290] - Human (A) and mouse (B) spleen tissue lysates with caspase-12 antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Caspase-12 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid peptide near the amino terminus of murine Caspase-12. The immunogen is located within the first 50 amino acids of Caspase-12.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 0.5 to 1 mg/ml.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Caspase-12 Peptide (NBP1-77290PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caspase-12 Antibody

  • Apoptosis Related Cysteine Protease
  • CASP 12
  • CASP12
  • CASP12P1
  • Caspase 12 pseudogene 1
  • Caspase12
  • Caspase-12
  • UNQ9415

Background

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-12.

Caspase-12 - activator of apoptosis via the ER stress response
Aside from their important role in apoptosis, caspases also play an important role in inflammatory processes. Humans express four inflammatory caspases: Caspase-1, -4, -5, and -12. Caspase-12 is a 48 kDa protein localized to the ER and involved in...  Read full blog post.

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Bioinformatics

Gene Symbol CASP12
Entrez
Uniprot