Caspase-12 Antibody

Western Blot: Caspase-12 Antibody [NBP1-77290] - Analysis of caspase-12 in (A) human and (B) mouse spleen tissue lysates with caspase-12 antibody at 1 ug/mL.

Immunohistochemistry: Caspase-12 Antibody [NBP1-77290] - Caspase-12 in human heart tissue with caspase-12 antibody at 10 ug/mL.

Product Discontinued

Caspase-12 Antibody Summary

• Immunogen: Antibody was raised against a 16 amino acid peptide near the amino terminus of murine Caspase-12. The immunogen is located within the first 50 amino acids of Caspase-12. Amino Acid Squence: AARRTHERDPIYKIKG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP12
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 0.5 to 1 mg/ml.
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Caspase-12 Antibody

• Apoptosis Related Cysteine Protease
• CASP 12
• CASP12
• CASP12P1
• Caspase 12 pseudogene 1
• Caspase12
• Caspase-12
• UNQ9415

Background

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP12
• Uniprot:
  • CAA73532(Mouse)