Casein Kinase 2 beta Antibody

Western Blot: Casein Kinase 2 beta Antibody [NBP1-06514] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.

Immunohistochemistry: Casein Kinase 2 beta Antibody [NBP1-06514] - Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-CKII beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .

Immunohistochemistry-Paraffin: Casein Kinase 2 beta Antibody [NBP1-06514] - FFPE section of human testicular seminoma. Affinity purified rabbit anti-CKII beta used at a dilution of 1:250.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Casein Kinase 2 beta Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human casein kinase 2, beta using the numbering given in entry NP_001311.3 (GeneID 1460).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSNK2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  Casein Kinase 2 beta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Casein Kinase 2 beta Antibody

• Casein Kinase 2 beta
• casein kinase 2, beta polypeptide
• Casein kinase II beta subunit
• casein kinase II subunit beta
• CK II beta
• CK2B
• CK2N
• CSK2B
• CSNK2B
• G5A
• MGC138222
• MGC138224
• Phosvitin
• Protein G5a

Background

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSNK2B
• Entrez:
  • 1460(Human)
  • 13001(Mouse)
• Uniprot:
  • NP_001311.3(Human)