Casein Kinase 1 epsilon Antibody

Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - (1ug/ml) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NB100-93388] - Staining of paraffin embedded human tonsil tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: Casein Kinase 1 epsilon Antibody [NB100-93388] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Casein Kinase 1 epsilon Antibody Summary

• Immunogen: Peptide with sequence C-PASQTSVPFDHLGK corresponding to C-Terminus according to NP_689407.1.
• Epitope: C-PASQTSVPFDHLGK
• Specificity: Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSNK1E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Control:

  Casein Kinase 1 epsilon Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Casein Kinase 1 epsilon Antibody

• Casein Kinase 1 epsilon
• casein kinase 1, epsilon
• casein kinase I isoform epsilon
• CKIe
• CKI-epsilon
• CSNK1E
• EC 2.7.11
• EC 2.7.11.1
• HCKIE
• MGC10398

Background

CKI epsilon is a member of the casein kinase 1 (CK1) serine/threonine kinase family of proteins. In mammals, there are seven distinct genes that represent the CK1 family: alpha, beta, gamma-1, gamma-2, gamma-3, delta, and epsilon. CKI epsilon phosphorylates a large number of proteins and is involved in pathways involving Wnt and AKT. CKI epsilon has been found to bind and phosphorylate the circadian protein, Period, and the Wnt signaling protein, Dsh. In the Wnt pathway, CKI epsilon activity is required for the full activation of Dsh and downstream signaling effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 1 epsilon Antibody (NB100-93388)(1)

Publication using NB100-93388

• Hammerlein A, Weiske J, Huber O. A second protein kinase CK1-mediated step negatively regulates Wnt signalling by disrupting the lymphocyte enhancer factor-1/beta-catenin complex. Cell Mol Life Sci 2005-03-01 [PMID: 15747065]

Bioinformatics

• Gene Symbol: CSNK1E
• Entrez:
  • 1454(Human)
  • 27373(Mouse)
  • 58822(Rat)
• Uniprot:
  • NP_001885.1(Human)