CARS Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining in human cerebral cortex and skeletal muscle tissues using anti-CARS antibody. Corresponding CARS RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human cerebral cortex, pancreas, skeletal muscle and testis using Anti-CARS antibody NBP1-86624 (A) shows similar ...read more
Independent Antibodies: Western Blot: CARS Antibody [NBP1-86624] - Analysis using Anti-CARS antibody NBP1-86624 (A) shows similar pattern to independent antibody NBP1-86623 (B).
Immunocytochemistry/ Immunofluorescence: CARS Antibody [NBP1-86624] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human testis.
Western Blot: CARS Antibody [NBP1-86624] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat ...read more
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human pancreas.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CARS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CARS Protein (NBP1-86624PEP)
Publications
Read Publication using NBP1-86624.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CARS Antibody

  • CARS1
  • CysRS
  • cysteine translase
  • cysteine tRNA ligase 1, cytoplasmic
  • Cysteine--tRNA ligase
  • cysteinyl-tRNA synthetase
  • cysteinyl-tRNA synthetase, cytoplasmic
  • EC 6.1.1
  • EC 6.1.1.16
  • MGC:11246

Background

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CARS Antibody (NBP1-86624)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CARS Antibody (NBP1-86624)

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Blogs on CARS

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol CARS