CARS Antibody

Images

 
Western Blot: CARS Antibody [NBP1-86624] - Analysis using Anti-CARS antibody NBP1-86624 (A) shows similar pattern to independent antibody NBP1-86623 (B).
Immunocytochemistry/ Immunofluorescence: CARS Antibody [NBP1-86624] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human testis.
Western Blot: CARS Antibody [NBP1-86624] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat ...read more
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human skeletal muscle shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining in human cerebral cortex and skeletal muscle tissues using anti-CARS antibody. Corresponding CARS RNA-seq data are ...read more
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human cerebral cortex, pancreas, skeletal muscle and testis using Anti-CARS antibody NBP1-86624 (A) shows similar protein distribution across ...read more
Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human pancreas.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

CARS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE
Specificity
Specificity of human, mouse, rat CARS antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CARS Protein (NBP1-86624PEP)
Publications
Read Publication using NBP1-86624.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CARS Antibody

  • CARS1
  • CysRS
  • cysteine translase
  • cysteine tRNA ligase 1, cytoplasmic
  • Cysteine--tRNA ligase
  • cysteinyl-tRNA synthetase
  • cysteinyl-tRNA synthetase, cytoplasmic
  • EC 6.1.1
  • EC 6.1.1.16
  • MGC:11246

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CARS Antibody (NBP1-86624)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CARS Antibody (NBP1-86624) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CARS Antibody (NBP1-86624)

Discover related pathways, diseases and genes to CARS Antibody (NBP1-86624). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CARS Antibody (NBP1-86624)

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Pathways for CARS Antibody (NBP1-86624)

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PTMs for CARS Antibody (NBP1-86624)

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Blogs on CARS

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol CARS