CARD10 Antibody - BSA Free

Western Blot: CARD10 Antibody [NBP1-76676] - Analysis of CARD10 in A-20 cell lysate with CARD10 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: CARD10 Antibody - BSA Free [NBP1-76676] - Immunofluorescence of CARD10 in EL4 cells with CARD10 antibody at 20 u/mL.

Immunocytochemistry/ Immunofluorescence: CARD10 Antibody - BSA Free [NBP1-76676] - Immunocytochemistry of CARD10 in EL4 cells with CARD10 antibody at 10 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CARD10 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10. The immunogen is located within the last 50 amino acids of CARD10. Amino Acid Squence: KECGSSRGCPSSSED
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARD10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 5 ug/ml
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  CARD10 Peptide (NBP1-76676PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CARD10 Antibody - BSA Free

• CARD-containing MAGUK 3 protein
• CARD-containing MAGUK protein 3
• Carma 3
• CARMA3BIMP1Bcl10 binding protein and activator of NFKB
• caspase recruitment domain family, member 10
• caspase recruitment domain-containing protein 10
• MGC142219

Background

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kappaB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkappaK complex, demonstrating its importance in the regulation of NF-kappaB transcription factor activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CARD10 Antibody (NBP1-76676)(1)

Publication using NBP1-76676

• Martin D, Galisteo R, Gutkind JS. CXCL8/IL8 stimulates vascular endothelial growth factor (VEGF) expression and the autocrine activation of VEGFR2 in endothelial cells by activating NFkappaB through the CBM (Carma3/Bcl10/Malt1) complex. J Biol Chem. 2009-03-06 [PMID: 19112107] (WB, Mouse)
  
  Details:
  WB (mouse SV40-immortalized endothelial cells), Fig. 4A,F.

Bioinformatics

• Gene Symbol: CARD10
• Uniprot:
  • AAK26165(Human)