CARD10 Antibody

Images

 
Western Blot: CARD10 Antibody [NBP1-76676] - Analysis of CARD10 in A-20 cell lysate with CARD10 antibody at 5 ug/mL.
Immunocytochemistry/ Immunofluorescence: CARD10 Antibody [NBP1-76676] - Immunofluorescence of CARD10 in EL4 cells with CARD10 antibody at 20 ug/mL.
Western Blot: CARD10 Antibody [NBP1-76676] - Analysis of CARD10 expression in EL4 cell lysate with CARD10 antibody using NBP1-76676 at (A) 5 and (B) 10 ug /ml.
Immunocytochemistry/ Immunofluorescence: CARD10 Antibody [NBP1-76676] - Immunocytochemistry of CARD10 in EL4 cells using NBP1-76676 at 10 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CARD10 Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10. The immunogen is located within the last 50 amino acids of CARD10.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARD10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
CARD10 antibody can be used for detection of CARD10 by Western blot at 5 - 10 mg/ml.
Theoretical MW
115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CARD10 Peptide (NBP1-76676PEP)
Publications
Read Publication using
NBP1-76676 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CARD10 Antibody

  • CARD-containing MAGUK 3 protein
  • CARD-containing MAGUK protein 3
  • Carma 3
  • CARMA3BIMP1Bcl10 binding protein and activator of NFKB
  • caspase recruitment domain family, member 10
  • caspase recruitment domain-containing protein 10
  • MGC142219

Background

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kappaB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkappaK complex, demonstrating its importance in the regulation of NF-kappaB transcription factor activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CARD10 Antibody (NBP1-76676)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Mouse
(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CARD10
Uniprot