Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric)

ELISA: Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) [NBP3-06962] - Samples were spiked with high concentrations of Mouse Carboxypeptidase B2/CPB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) [NBP3-06962] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse Carboxypeptidase B2/CPB2 in samples. No significant cross-reactivity or interference between Mouse Carboxypeptidase B2/CPB2 and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 4.97
• Intra-Assay: %CV < 5.21
• Spike Recovery: 87 - 111%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CPB2

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric)

• carboxypeptidase B2 (plasma)
• carboxypeptidase B2 (plasma, carboxypeptidase U)
• Carboxypeptidase B2
• Carboxypeptidase U
• CPB2
• CPU
• CPUEC 3.4.17.20
• EC 3.4.17
• pCPB
• Plasma carboxypeptidase B
• TAFI
• TAFIcarboxypeptidase B-like protein
• Thrombin-activable fibrinolysis inhibitor
• thrombin-activatable fibrinolysis inhibitor

Background

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Available sequence data analyses indicate splice variants that encode different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Carboxypeptidase B2/CPB2 ELISA Kit (NBP3-06962). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CPB2