CaMKII beta Antibody

Images

 
Western Blot: CaMKII beta Antibody [NBP1-88212] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NBP1-88212] - Staining of mouse somatosensory cortex shows immunoreactivity in neuronal cells bodies and dendrites.
Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP1-88212] - Staining of human cerebral cortex shows positivity in a subset of neurons.
Western Blot: CaMKII beta Antibody [NBP1-88212] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Cerebral Cortex
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NBP1-88212] - Staining of mouse cerebellum shows positivity in neurons.
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NBP1-88212] - Staining of mouse forebrain shows positivity in cell bodies and processes of neurons in ventral pallidum and piriform cortex.
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NBP1-88212] - Staining of mouse medulla shows immunoreactivity in the inferior olivary nucleus and raphe obscures nucleus.
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NBP1-88212] - Staining of mouse olfactory bulb shows positivity in neuronal cell bodies and processes in anterior olfactory nucleus.
Immunohistochemistry: CaMKII beta Antibody [NBP1-88212] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP1-88212] - Staining of human cerebellum shows positivity in Purkinje cells as well as in molecular and granular cell layers
Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP1-88212] - Staining of human cerebral cortex shows moderate immunoreactivity in neuropil and cytoplasmic positivity in neuronal cell bodies.
Simple Western: CaMKII beta Antibody [NBP1-88212] - Simple Western lane view shows a specific band for CaMKII beta in 0.2 mg/ml of H. Motor Cortex lysate(s). This experiment was performed under reducing conditions using ...read more
Simple Western: CaMKII beta Antibody [NBP1-88212] - Electropherogram image of the corresponding Simple Western lane view. CaMKII beta antibody was used at 1:5 dilution on H. Motor Cortex lysate(s) respectively.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CaMKII beta Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:TRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIH
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Simple Western 1:5
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. WB dilution: 1:100-1:500 (Rodent material) and 1:500-1:1000 (Human material). In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control Peptide
CaMKII beta Protein (NBP1-88212PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CaMKII beta Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II beta
  • calcium/calmodulin-dependent protein kinase II beta
  • calcium/calmodulin-dependent protein kinase type II beta chain
  • calcium/calmodulin-dependent protein kinase type II subunit beta
  • CaM kinase II beta subunit
  • CaM kinase II subunit beta
  • CAM2CAMK2
  • CAMKB
  • CaMK-II subunit beta
  • CaM-kinase II beta chain
  • EC 2.7.11
  • EC 2.7.11.17
  • MGC29528
  • proline rich calmodulin-dependent protein kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CAMK2B