CaM Kinase II delta Antibody (438422)

Western blot shows lysates of human heart, liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CaM Kinase IId Monoclonal Antibody (Catalog # MAB4176) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CaM Kinase IId isoforms at approximately 50 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu
• Applications: WB
• Clone: 438422
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CaM Kinase II delta Antibody (438422) Summary

• Immunogen: E. coli-derived recombinant human CaM Kinase IIδ
  Ala402-Lys478
  Accession # Q13557
• Specificity: Detects endogenous human CaM Kinase IIδ isoforms in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKII alpha , CaMKII beta , or CaMKII gamma .
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAMK2D
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CaM Kinase II delta Antibody (438422)

• calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
• calcium/calmodulin-dependent protein kinase II delta
• calcium/calmodulin-dependent protein kinase type II delta chain
• calcium/calmodulin-dependent protein kinase type II subunit delta
• CaM kinase II delta subunit
• CaM Kinase II delta
• CaM kinase II subunit delta
• CAMK2D
• CAMKD
• CaMK-II delta subunit
• CaMK-II subunit delta
• CaM-kinase II delta chain
• DKFZp686G23119
• DKFZp686I2288
• EC 2.7.11
• EC 2.7.11.17
• MGC44911

Background

Calcium/calmodulin-dependent protein kinase type II delta (CaMKIIδ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha , beta , gamma , and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIIδ expression is ubiquitous and has been identified as the predominant CaMKII isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaM Kinase II delta Antibody (MAB4176)(5)

Publications using MAB4176

• Cachorro E, G�nscht M, Schubert M et al. CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2 Circulation research 2023-02-17 [PMID: 36715019]
• Michael Wagner, Mirna S. Sadek, Nataliya Dybkova, Fleur E. Mason, Johann Klehr, Rebecca Firneburg et al. Cellular Mechanisms of the Anti-Arrhythmic Effect of Cardiac PDE2 Overexpression International Journal of Molecular Sciences 2021-05-01 [PMID: 34062838]
• Beauchemin M, Geguchadze R, Guntur AR, et al. Exploring mechanisms of increased cardiovascular disease risk with antipsychotic medications: Risperidone alters the cardiac proteomic signature in mice Pharmacol. Res. 12/23/2019 [PMID: 31874253]
• Sadegh MK, Ekman M, Rippe C, Uvelius B, Sward K, Albinsson S Deletion of Dicer in smooth muscle affects voiding pattern and reduces detrusor contractility and neuroeffector transmission. PLoS ONE, 2012-04-27;7(4):e35882. 2012-04-27 [PMID: 22558254] (Western Blot, Mouse)
• Turczynska KM, Sadegh MK, Hellstrand P, Sward K, Albinsson S MicroRNAs are essential for stretch-induced vascular smooth muscle contractile differentiation via microRNA (miR)-145-dependent expression of L-type calcium channels. J. Biol. Chem., 2012-04-02;287(23):19199-206. 2012-04-02 [PMID: 22474293] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CAMK2D
• Uniprot:
  • Q13557()