CaM Kinase II delta Antibody (10M1P2)

Western Blot: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Western blot analysis of extracts of various cell lines, using CaM Kinase II delta Rabbit mAb (NBP3-16783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Immunofluorescence analysis of NIH-3T3 cells using CaM Kinase II delta Rabbit mAb (NBP3-16783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Immunofluorescence analysis of C6 cells using CaM Kinase II delta Rabbit mAb (NBP3-16783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (10M1P2) [NBP3-16783] - Confocal imaging of NIH/3T3 cells using CaM Kinase II delta Rabbit mAb ollowed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 10M1P2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CaM Kinase II delta Antibody (10M1P2) Summary

• Description: Novus Biologicals Rabbit CaM Kinase II delta Antibody (10M1P2) (NBP3-16783) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CaM Kinase II delta (Q13557). MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFE
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAMK2D
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CaM Kinase II delta Antibody (10M1P2)

• calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
• calcium/calmodulin-dependent protein kinase II delta
• calcium/calmodulin-dependent protein kinase type II delta chain
• calcium/calmodulin-dependent protein kinase type II subunit delta
• CaM kinase II delta subunit
• CaM Kinase II delta
• CaM kinase II subunit delta
• CAMK2D
• CAMKD
• CaMK-II delta subunit
• CaMK-II subunit delta
• CaM-kinase II delta chain
• DKFZp686G23119
• DKFZp686I2288
• EC 2.7.11
• EC 2.7.11.17
• MGC44911

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Four alternatively spliced transcript variants that encode three different isoforms have been characterized to date. Distinct isoforms of this chain have different expression patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK2D