CaM Kinase II alpha Antibody

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Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa ...read more
Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain ...read more
Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa ...read more
Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval withcitrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CaM Kinase II alpha Antibody Summary

Immunogen
Peptide with sequence C-PRTAQSEETRVWHR corresponding to internal region according to NP_741960.1.
Epitope
C-PRTAQSEETRVWHR
Specificity
This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CAMK2A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 65 kDa band bands observed in human brain (cerebellum) and mouse and rat brain lysates and an additional 50 kDa band in the rodent brain lysates (calculated MW of 55.3 kDa band according to human NP_057065.2 and of 54.1 according to human NP_741960.1 to mouse NP_803126.1 and to rat NP_037052.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. IHC-P: Human brain cortex shows granular staining in the periphery of pyramydal cell bodies.
Reviewed Applications
Read 1 Review rated 4
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NBP1-51945 in the following applications:

Publications
Read Publication using NBP1-51945.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CaM Kinase II alpha Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM Kinase II alpha
  • CaM kinase II subunit alpha
  • CAMK2A
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-51945
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA

Publications for CaM Kinase II alpha Antibody (NBP1-51945)(1)

Review for CaM Kinase II alpha Antibody (NBP1-51945) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-51945:
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IHC
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry CaM Kinase II alpha NBP1-51945
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reviewed by:
Hayk Simonyan
IHC Mouse 04/30/2018
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Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
LotP1E260218

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Hayk Simonyan
04/30/2018
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot