Calsequestrin 1 Antibody

Western Blot: Calsequestrin 1 Antibody [NB120-3516] - Analysis of 25 ug of RD (Lane 1), L6 (Lane 2) and mouse heart (Lane 3) cell lysates.

Immunocytochemistry/Immunofluorescence: Calsequestrin 1 Antibody [NB120-3516] - Analysis of Calsequestrin (green) showing staining in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NB120-3516] - Analysis showing positive staining in the cytoplasm of Human heart tissue (right) compared with a negative control in the absence of primary antibody (left).

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NB120-3516] - Analysis showing positive staining in the cytoplasm of paraffin-treated Mouse heart tissue (right) compared with a negative control in the absence of primary antibody (left).

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NB120-3516] - Analysis showing positive staining in the cytoplasm of Human skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).

Product Discontinued

Calsequestrin 1 Antibody Summary

• Immunogen: Purified canine cardiac calsequestrin.
• Localization: Isoform 1: Sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial Isoform 2: Smooth endoplasmic reticulum.
• Specificity: This detects Calsequestrin. It recognizes both cardiac and skeletal muscle Calsequestrin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASQ1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000 - 1:10000
• Application Notes: ICC/IF usage was reported in scientific literature. WB: Detects an approx. 55 kDa protein representing Calsequestrin from canine cardiac extract. Additional bands at 97 kDa may be observed and have been reported to be Calsequestrin-like proteins.
• Control:

  Calsequestrin 1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole serum diluted in PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Calsequestrin 1 Antibody

• calmitin
• calmitine
• calsequestrin 1 (fast-twitch, skeletal muscle)
• Calsequestrin, skeletal muscle isoform
• calsequestrin-1
• CASQ
• PDIB1

Background

The sarcoplasmic reticulum (SR) is in part responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium. Two mammalian isoforms of calequestrin exist, a cardiac and a skeletal muscle isoform, which are the products of different genes and are 63% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calsequestrin 1 Antibody (NB120-3516)(3)

Publications using NB120-3516

• Castillero E, Akashi H, Pendrak K et al. Attenuation of the Unfolded Protein Response and Endoplasmic Reticulum Stress after Mechanical Unloading in Dilated Cardiomyopathy. Am J Physiol Heart Circ Physiol 2015-06-08 [PMID: 26055788]
• Lai NC, Tang T, Gao MH et al. Improved function of the failing rat heart by regulated expression of insulin-like growth factor I via intramuscular gene transfer Hum Gene Ther 2012-03-01 [PMID: 22017392]
• Shankar J, Messenberg A, Chan J et al. Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells Cancer Res 2010-05-01 [PMID: 20388789] (ICC/IF, Human)

FAQs for Calsequestrin 1 Antibody (NB120-3516). (Showing 1 - 1 of 1 FAQs).

1. For catalog #NB120-3516, do you have any additional data or an image(s) available for use of this antibody specifically with mouse samples in WB?
   • I unfortunately do not have any additional images for mouse samples on Western Blot to send over to you as we have had this reactivity reported to us by customers. As such I do not have their data on file, but we are extremely confident it will work in mouse samples expressing the protein in Western Blot. Of course we do have a 100% guarantee so if you do not get it to work we are happy to send you a 100% refund or replacement.

Bioinformatics

• Gene Symbol: CASQ1
• Entrez:
  • 844(Human)
  • 12372(Mouse)
  • 686019(Rat)