Calreticulin-2/CALR3 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Calreticulin-2/CALR3 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Calreticulin-2
  Thr20-Leu384
  Accession # Q96L12
• Specificity: Detects human Calreticulin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Calreticulin-1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calreticulin-2/CALR3 Antibody [Alexa Fluor® 647]

• CALR3
• calreticulin 2
• calreticulin 3
• Calreticulin2
• Calreticulin-2
• calreticulin-3
• cancer/testis antigen 93
• Crt2
• CRT2calreticulin-2
• CT93
• FLJ25355
• MGC26577

Background

Calreticulin-2 is a testis-specific, soluble, lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca⁺⁺ ion with high affinity, and the C-terminus binds Ca⁺⁺ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin-2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.