Calreticulin-2/CALR3 Antibody

MCF‑7 human breast cancer cell line was stained with Goat Anti-Human Calreticulin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2927, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Calreticulin-2/CALR3 Antibody Summary

• Immunogen: E. coli-derived recombinant human Calreticulin-2
  Thr20-Leu384
  Accession # Q96L12
• Specificity: Detects human Calreticulin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Calreticulin-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CALR3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calreticulin-2/CALR3 Antibody

• CALR3
• calreticulin 2
• calreticulin 3
• Calreticulin2
• Calreticulin-2
• calreticulin-3
• cancer/testis antigen 93
• Crt2
• CRT2calreticulin-2
• CT93
• FLJ25355
• MGC26577

Background

Calreticulin-2 is a testis-specific, soluble, lectin-like molecule that participates in the protein folding and quality control cycle in the endoplasmic reticulum (ER) lumen. Mature human Calreticulin-2 has a 178 amino acid N-terminal globular domain, a 97 aa central P-domain, and a 90 aa C-terminal domain. The globular domain shows lectin activity, the P-domain binds one Ca⁺⁺ ion with high affinity, and the C-terminus binds Ca⁺⁺ and contains an RNEL ER retention motif. The amino acid sequence of Calreticulin-2 is 84%, 83% and 87% identical to that of mouse, bovine and canine Calreticulin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calreticulin-2/CALR3 Antibody (AF2927)(1)

Publication using AF2927

• Dijana Djureinovic, Björn M. Hallström, Masafumi Horie, Johanna Sofia Margareta Mattsson, Linnea La Fleur, Linn Fagerberg et al. Profiling cancer testis antigens in non–small-cell lung cancer JCI Insight 2016-10-05 [PMID: 27699219]

Bioinformatics

• Gene Symbol: CALR3
• Uniprot:
  • Q96L12()