Calponin 2 Antibody

Western Blot: Calponin 2 Antibody [NBP1-36741] - Analysis of Calponin 2 in HepG2 lysates (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using this antibody at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Calponin 2 Antibody [NBP1-36741] - (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Calponin 2 Antibody Summary

• Description: Novus Biologicals Goat Calponin 2 Antibody (NBP1-36741) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Calponin 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DPGEVPEYPPYYQEE corresponding to internal region (near C-Terminus) according to NP_004359.1, NP_958434.1.
• Epitope: internal region (near C Terminus)
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in HepG2 lysates (calculated MW of 33.7 kDa band according to NP_004359.1).Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Placenta.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Calponin 2 Antibody

• calponin 2
• Calponin H2, smooth muscle
• calponin-2
• Neutral calponin

Background

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calponin 2 Antibody (NBP1-36741)(1)

Publication using NBP1-36741

• Masuda H, Tanaka K, Takagi M et al. Molecular cloning and characterization of human non-smooth muscle calponin. J Biochem 1996-08-01 [PMID: 8889829]

Bioinformatics

• Gene Symbol: CNN2
• Entrez:
  • 1265(Human)
• Uniprot:
  • NP_004359.1(Human)