Calponin 2 Antibody (C5) - BSA Free

Western Blot: Calponin 2 Antibody (C5) [NBP3-41851] - Sample: HepG2 cell lysate Primary Ab: 2ug/ml NBP3-41851; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

• Reactivity: Hu
• Applications: WB
• Clone: C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Calponin 2 Antibody (C5) - BSA Free Summary

• Description: Novus Biologicals Mouse Calponin 2 Antibody (C5) - BSA Free (NBP3-41851) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunized with recombinant Human Calponin 2 (Uniprot#: Q99439; Met1-Gln121 + Ala131-Tyr269 (Uniprot#: Q99439)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNN2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01M PBS, pH7.4, 50% glycerol
• Preservative: 0.05% Proclin 300
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for Calponin 2 Antibody (C5) - BSA Free

• calponin 2
• Calponin H2, smooth muscle
• calponin-2
• Neutral calponin

Background

Calponin, a 34 kDa protein, regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an actin- and tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-actin and inhibition of the actin-activated MgATPase activity of phosphorylated myosin.Both properties are lost following phosphorylation (primarily at serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin), and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNN2