Calponin 2 Antibody (4C9) - BSA Free

Western Blot: Calponin 2 Antibody (4C9) [NBP3-42944] - pig stomach tissue were subjected to SDS PAGE followed by western blot with (Calponin 2 Antibody) at dilution of 1:2000

Immunohistochemistry: Calponin 2 Antibody (4C9) [NBP3-42944] - Immunohistochemistry of paraffin-embedded human breast hyperplasia tissue slide using (Calponin 2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

• Reactivity: Hu, Po
• Applications: WB, ELISA, IHC
• Clone: 4C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Calponin 2 Antibody (4C9) - BSA Free Summary

• Description: Novus Biologicals Mouse Calponin 2 Antibody (4C9) - BSA Free (NBP3-42944) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: calponin 2 - (Uniprot# Q99439)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNN2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:500 - 1:4000
  • Western Blot 1:1000 - 1:4000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Calponin 2 Antibody (4C9) - BSA Free

• calponin 2
• Calponin H2, smooth muscle
• calponin-2
• Neutral calponin

Background

Calponin, a 34 kDa protein, regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an actin- and tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-actin and inhibition of the actin-activated MgATPase activity of phosphorylated myosin.Both properties are lost following phosphorylation (primarily at serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin), and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNN2