Calponin 1 Antibody (SI67-01)

Western Blot: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Western blot analysis of Calponin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Ant

Immunocytochemistry/Immunofluorescence: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Staining Calponin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Calponin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Analysis of Calponin on Hela cell lysates using anti-Calponin antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Analysis of paraffin-embedded human breast tissue using anti-Calponin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Analysis of paraffin-embedded mouse bladder tissue using anti-Calponin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Analysis of paraffin-embedded mouse heart tissue using anti-Calponin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SI67-01) [NBP2-67401] - Analysis of paraffin-embedded mouse stomach tissue using anti-Calponin antibody. Counter stained with hematoxylin.

Product Discontinued

Calponin 1 Antibody (SI67-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Calponin 1 aa 248-297 / 297. (SwissProt: P51911 Human; SwissProt: Q08091 Mouse; SwissProt: Q08290 Rat)
• Localization: Cytoskeleton, focal adhesion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CNN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Calponin 1 Antibody (SI67-01)

• Basic calponin
• Calponin 1
• calponin 1, basic, smooth muscle
• Calponin H1
• Calponin H1, smooth muscle
• calponins, basic
• CNN1
• Sm-Calp
• SMCC
• SMCCcalponin-1

Background

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calponin 1 Antibody (NBP2-67401)(1)

Publication using NBP2-67401

• Lin CJ, Staiculescu MC, Hawes JZ et al. Heterogeneous Cellular Contributions to Elastic Laminae Formation in Arterial Wall Development Circ. Res. 2019-10-08 [PMID: 31590613]

Bioinformatics

• Gene Symbol: CNN1