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Rat Calpain 1 ELISA Kit (Colorimetric)

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Rat Calpain 1 ELISA Kit (Colorimetric) - Standard Curve Reference
Rat Calpain 1 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

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Rat Calpain 1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CAPN1 in samples. No significant cross-reactivity or interference between Rat CAPN1 and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.27%
Intra-Assay
CV% < 5.47%
Spike Recovery
86-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CAPN1

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Substrate Reagent
  2. Stop Solution
  3. Micro ELISA Plate(Dismountable)
  4. HRP Conjugate Diluent
  5. Reference Standard & Sample Diluent
  6. Reference Standard
  7. Plate Sealer
  8. Product Description
  9. Concentrated Biotinylated Detection Ab (100×)
  10. Certificate of Analysis
  11. Concentrated HRP Conjugate (100×)
  12. Concentrated Wash Buffer (25×)
  13. Biotinylated Detection Ab Diluent

Alternate Names for Rat Calpain 1 ELISA Kit (Colorimetric)

  • Calcium-activated neutral proteinase 1
  • calpain 1, (mu/I) large subunit
  • Calpain mu-type
  • calpain, large polypeptide L1
  • calpain-1 catalytic subunit
  • Calpain-1 large subunit
  • CANP 1
  • CANP
  • CANPL1EC 3.4.22.52
  • Cell proliferation-inducing gene 30 protein
  • cell proliferation-inducing protein 30
  • EC 3.4.22
  • micromolar-calpain
  • muCANPCANP1
  • muCL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.
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Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
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Western Blot: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of CAPNS1 expression in transfected 293T cell line by CAPNS1 monoclonal antibody (M01), clone 3C4.Lane 1: CAPNS1 transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to CAPNS1 on HeLa cell. Antibody concentration 20 ug/ml.
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Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
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Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.
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Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
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Western Blot: Calmodulin 2 Antibody [NBP2-14871] - Sample (50 ug of whole cell lysate) A: Mouse brain 15% SDS PAGE; antibody diluted at 1:1000.
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Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.
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Western Blot: PRG2 Antibody [NBP1-88573] - Analysis in control (vector only transfected HEK293T lysate) and pRG2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PRG2 Antibody [NBP1-88573] - Staining of human skeletal muscle shows no cytoplasmic positivity as expected.
Immunohistochemistry-Paraffin: PRG2 Antibody [NBP1-88573] - Staining of human placenta shows moderate to strong cytoplasmic positivity in extra villous trophoblast.
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Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
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Bioinformatics

Gene Symbol CAPN1