Human Calpain 1 ELISA Kit (Colorimetric)

ELISA: Human Calpain 1 ELISA Kit (Colorimetric) [NBP2-74979] - Samples were spiked with high concentrations of Human Calpain 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Calpain 1 ELISA Kit (Colorimetric) [NBP2-74979] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Calpain 1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CAPN1 in samples. No significant cross-reactivity or interference between Human CAPN1 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.43%
• Intra-Assay: CV% < 4.48%
• Spike Recovery: 85-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CAPN1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Calpain 1 ELISA Kit (Colorimetric)

• Calcium-activated neutral proteinase 1
• Calpain 1
• calpain 1, (mu/I) large subunit
• Calpain mu-type
• calpain, large polypeptide L1
• calpain-1 catalytic subunit
• Calpain-1 large subunit
• CANP 1
• CANP
• CANPL1
• CANPL1EC 3.4.22.52
• CAPN1
• Cell proliferation-inducing gene 30 protein
• cell proliferation-inducing protein 30
• EC 3.4.22
• EC 3.4.22.52
• Micromolar-calpain
• muCANP
• muCANPCANP1
• muCL
• PIG30

Background

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Calpain 1 ELISA Kit (NBP2-74979). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CAPN1