Calpain 1 Antibody (CAPN1/1530) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: CAPN1/1530
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Calpain 1 Antibody (CAPN1/1530) [Alexa Fluor® 594] Summary

• Immunogen: Recombinant full-length human Calpain 1 protein (Uniprot: P07384)
• Localization: Cytoplasm and Cell Surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAPN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Calpain 1 Antibody (CAPN1/1530) [Alexa Fluor® 594]

• Calcium-activated neutral proteinase 1
• Calpain 1
• calpain 1, (mu/I) large subunit
• Calpain mu-type
• calpain, large polypeptide L1
• calpain-1 catalytic subunit
• Calpain-1 large subunit
• CANP 1
• CANP
• CANPL1
• CANPL1EC 3.4.22.52
• CAPN1
• Cell proliferation-inducing gene 30 protein
• cell proliferation-inducing protein 30
• EC 3.4.22
• EC 3.4.22.52
• Micromolar-calpain
• muCANP
• muCANPCANP1
• muCL
• PIG30

Background

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAPN1