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Calpain 1 Antibody (107-82)

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Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ...read more
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ...read more
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
Clone
107-82
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Calpain 1 Antibody (107-82) Summary

Immunogen
Purified bovine skeletal muscle 80 kDa subunit of m-calpain.
Epitope
This antibody recognizes an epitope between amino acids 502-699 (domain III / IV) of human M Calpain.
Localization
Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Specificity
Detects the 80 kDa subunit of M Calpain in human platelets and erythrocytes; bovine platelets, heart and skeletal muscle; rat myoblasts, kidney, liver and spleen; and cultured pig cells. This does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAPN1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen 1:50
Application Notes
WB: Detects an approx. 80 kDa protein representing M Calpain in bovine platelets. ICC: Staining of M Calpain in LLC-PK1 cells results in diffuse cytoplasmic staining.
Control
Calpain 1 Overexpression Lysate
Calpain 1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB120-3588 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Calpain 1 Antibody (107-82)

  • Calcium-activated neutral proteinase 1
  • calpain 1, (mu/I) large subunit
  • Calpain mu-type
  • calpain, large polypeptide L1
  • calpain-1 catalytic subunit
  • Calpain-1 large subunit
  • CANP 1
  • CANP
  • CANPL1EC 3.4.22.52
  • Cell proliferation-inducing gene 30 protein
  • cell proliferation-inducing protein 30
  • EC 3.4.22
  • micromolar-calpain
  • muCANPCANP1
  • muCL

Background

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Calpastatin Antibody (1F7E3D10) [NB120-5582] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
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Western Blot: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of CAPNS1 expression in transfected 293T cell line by CAPNS1 monoclonal antibody (M01), clone 3C4.Lane 1: CAPNS1 transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Calpain small subunit 1 Antibody (3C4) [H00000826-M01] - Analysis of monoclonal antibody to CAPNS1 on HeLa cell. Antibody concentration 20 ug/ml.
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Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
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Immunohistochemistry-Paraffin: PRG2 Antibody [NBP1-88573] - Staining of human placenta shows moderate to strong cytoplasmic positivity in extra villous trophoblast.
(1 Publication)
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Publications for Calpain 1 Antibody (NB120-3588) (0)

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Review for Calpain 1 Antibody (NB120-3588) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: MOUSE.

Reviews using NB120-3588:
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Western Blot Calpain 1 NB120-3588
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reviewed by:
Anonymous
 WB MOUSE 06/14/2019
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Summary

ApplicationWestern Blot
Sample Tested primary neonatal ventricular cardiomyocytes
SpeciesMOUSE
LotRH241730

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Control Lysate(s)

Calpain 1 Overexpression Lysate
Calpain 1 Knockout HeLa Cell Lysate

Secondary Antibodies

 

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Western Blot Calpain 1 NB120-3588
Enlarge
 Anonymous
06/14/2019
Application: WB
Species: MOUSE
read this review

Bioinformatics

Gene Symbol CAPN1
Entrez