Calpain 1 Antibody (107-82)

Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody (107-82) [NB120-3588] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

• Reactivity: Hu, Mu, Rt, Po, Bv, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
• Clone: 107-82
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Calpain 1 Antibody (107-82) Summary

• Immunogen: Purified bovine skeletal muscle 80 kDa subunit of m-calpain.
• Epitope: This antibody recognizes an epitope between amino acids 502-699 (domain III / IV) of human M Calpain.
• Localization: Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
• Specificity: Detects the 80 kDa subunit of M Calpain in human platelets and erythrocytes; bovine platelets, heart and skeletal muscle; rat myoblasts, kidney, liver and spleen; and cultured pig cells. This does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAPN1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Frozen 1:50
• Application Notes: WB: Detects an approx. 80 kDa protein representing M Calpain in bovine platelets. ICC: Staining of M Calpain in LLC-PK1 cells results in diffuse cytoplasmic staining.
• Positive Control:

  Calpain 1 Lysate (NBL1-08677)
  Calpain 1 Lysate (NBP2-65307)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Calpain 1 Antibody (107-82)

• Calcium-activated neutral proteinase 1
• calpain 1, (mu/I) large subunit
• Calpain mu-type
• calpain, large polypeptide L1
• calpain-1 catalytic subunit
• Calpain-1 large subunit
• CANP 1
• CANP
• CANPL1EC 3.4.22.52
• Cell proliferation-inducing gene 30 protein
• cell proliferation-inducing protein 30
• EC 3.4.22
• micromolar-calpain
• muCANPCANP1
• muCL

Background

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAPN1
• Entrez:
  • 823(Human)
  • 12333(Mouse)
  • 29153(Rat)