Calnexin Antibody (SN20-54)

Western Blot: Calnexin Antibody (SN20-54) [NBP2-67721] - Western blot analysis of Calnexin on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (SN20-54) [NBP2-67721] - Staining Calnexin in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Calnexin Antibody (SN20-54) [NBP2-67721] - Analysis of paraffin-embedded rat pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.

Flow Cytometry: Calnexin Antibody (SN20-54) [NBP2-67721] - Flow cytometric analysis of Calnexin was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: Calnexin Antibody (SN20-54) [NBP2-67721] - Analysis of Calnexin on Hela cells lysates using anti-Calnexin antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (SN20-54) [NBP2-67721] - Staining Calnexin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (SN20-54) [NBP2-67721] - Staining Calnexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Calnexin Antibody (SN20-54) [NBP2-67721] - Analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calnexin Antibody (SN20-54) [NBP2-67721] - Analysis of paraffin-embedded human liver cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calnexin Antibody (SN20-54) [NBP2-67721] - Analysis of paraffin-embedded human pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calnexin Antibody (SN20-54) [NBP2-67721] - Analysis of paraffin-embedded rat heart tissue using anti-Calnexin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calnexin Antibody (SN20-54) [NBP2-67721] - Analysis of paraffin-embedded rat kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.

Product Discontinued

Calnexin Antibody (SN20-54) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Calnexin aa 543-592 / 592. (SwissProt: P27824 Human; SwissProt: P35565 Rat)
• Localization: Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CANX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Calnexin Antibody (SN20-54)

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Calnexin, an abundant approx. 90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CANX