Caldesmon/CALD1 Antibody (E89)

Images

 
Western Blot: Caldesmon [p Ser789] Antibody (E89) [NB110-55647] - Western blot analysis on NIH3T3 cell lysate using anti-Caldesmon (NB110-55647); dilution 1:20,000.
Immunocytochemistry/ Immunofluorescence: Caldesmon [p Ser789] Antibody (E89) [NB110-55647] - HeLa cells.
Immunohistochemistry: Caldesmon/CALD1 [p Ser789] Antibody (E89) [NB110-55647] - Caldesmon (E89) on adenosis of breast
Immunohistochemistry: Caldesmon [p Ser789] Antibody (E89) [NB110-55647] - Immunohistochemical analysis of paraffin-embedded human uterus using anti-Caldesmon

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB110-55647
    • Availability
      Product Discontinued

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Caldesmon/CALD1 Antibody (E89) Summary

Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon was used as immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CALD1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-10000
  • Flow Cytometry 1:20
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 1:80
Application Notes
This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)

Reactivity Notes

Mouse cross reactivity tested by western blot and Immunohistochemistry. Rat cross reactivity tested by Western Blot only.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant in buffered aqueous solution
Preservative
No Preservative
Concentration
0.01 mg/ml
Purity
Protein A or G purified

Alternate Names for Caldesmon/CALD1 Antibody (E89)

  • CAD
  • CALD1
  • caldesmon 1
  • Caldesmon
  • CDM
  • CDMH-CAD
  • HCAD
  • LCAD
  • L-CAD
  • MGC21352
  • NAG22

Background

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Caldesmon/CALD1 Products

Bioinformatics Tool for Caldesmon/CALD1 Antibody (NB110-55647)

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Diseases for Caldesmon/CALD1 Antibody (NB110-55647)

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PTMs for Caldesmon/CALD1 Antibody (NB110-55647)

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Bioinformatics

Gene Symbol CALD1
Entrez
Uniprot