Recombinant Human Calcium Activated Nucleotidase 1/CANT1 Protein

Product Discontinued

Recombinant Human Calcium Activated Nucleotidase 1/CANT1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 63-401 of Human Calcium Activated Nucleotidase 1/CANT1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMRPAPG RPPTHNAHNW RLGQAPANWY NDTYPLSPPQ RTPAGIRYRI AVIADLDTES RAQEENTWFS YLKKGYLTLS DSGDKVAVEW DKDHGVLESH LAEKGRGMEL SDLIVFNGKL YSVDDRTGVV YQIEGSKAVP WVILSDGDGT VEKGFKAEWL AVKDERLYVG GLGKEWTTTT GDVVNENPEW VKVVGYKGSV DHENWVSNYN ALRAAAGIQP PGYLIHESAC WSDTLQRWFF LPRRASQERY SEKDDERKGA NLLLSASPDF GDIAVSHVGA VVPTHGFSSF KFIPNTDDQI IVALKSEEDS GRVASYIMAF TLDGRFLLPE TKIGSVKYEG IEFI

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CANT1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 40.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 50 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Calcium Activated Nucleotidase 1/CANT1 Protein

• Apyrase homolog
• calcium activated nucleotidase 1
• CANT1
• DBQD
• EC 3.6.1.6
• Putative MAPK-activating protein PM09
• Putative NF-kappa-B-activating protein 107
• SCAN1
• SCAN-1Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase
• SHAPY
• SHAPYmicromelic dwarfism with vertebral and metaphyseal abnormalities and advancedcarpotarsal ossification
• soluble Ca-activated nucleotidase, isozyme 1
• soluble calcium-activated nucleotidase 1
• soluble calcium-activated nucleotidase SCAN-1

Background

CANT1 (calcium-activated nucleotidase 1) belongs to the apyrase family. This protein is calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Also, it has very low activity towards ADP and even lower activity towards ATP. And it does not hydrolyze AMP and GMP. The specific function of CANT1 is as yet unknown, but its substrates are involved in several major signaling functions, including Ca2+ release, through activation of pyrimidinergic signaling. Recombinant human CANT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CANT1