Calcium Activated Nucleotidase 1/CANT1 Antibody (861206) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 861206
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Calcium Activated Nucleotidase 1/CANT1 Antibody (861206) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Calcium Activated Nucleotidase 1/CANT1
  Gly80-Ile401
  Accession # Q8WVQ1
• Specificity: Detects human Calcium Activated Nucleotidase 1/CANT1 in ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calcium Activated Nucleotidase 1/CANT1 Antibody (861206) [Alexa Fluor® 594]

• Apyrase homolog
• calcium activated nucleotidase 1
• CANT1
• DBQD
• EC 3.6.1.6
• Putative MAPK-activating protein PM09
• Putative NF-kappa-B-activating protein 107
• SCAN1
• SCAN-1Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase
• SHAPY
• SHAPYmicromelic dwarfism with vertebral and metaphyseal abnormalities and advancedcarpotarsal ossification
• soluble Ca-activated nucleotidase, isozyme 1
• soluble calcium-activated nucleotidase 1
• soluble calcium-activated nucleotidase SCAN-1

Background

CANT1 (soluble Calcium-Activated NucleoTidase 1; also SCAN-1, Apyrase homolog and NF kappa B-activating protein 107) is a 45-48 kDa glycoprotein member of the apyrase (ADP/ATP hydrolysing) family of molecules. It is an ER and Golgi-embedded protein that hydrolyzes UDP in a calcium-dependent manner, and appears to be involved in the regulation of protein glycosylation and folding. It is also reported that CANT1 is essential for cell proliferation. CANT1 is not ubiquitous, but is found in multiple cell types, including prostatic epithelium, fibroblasts, lymphocytes and chondrocytes. Human CANT1 is a 401 amino acid (aa) type II transmembrane glycoprotein. It contains a 44 aa N-terminal cytoplasmic region that possesses an ER retention motif (aa 38-42), and a 339 aa luminal domain (aa 63-401). CANT1 is reported to form membrane-bound disulfide-linked homodimers, and potentially forms non-covalent homodimers in a soluble (circulating) state. The soluble form is 35‑40 kDa in size, and presumably cleaved near the transmembrane segment. However, multiple splice forms are possible, and may account for the lower MWs. Two splice forms impact the extracellular region, one which contains a four aa substitution for aa 219-401, and a second that possesses a 26 aa substitution for aa 220‑401. Two additional splice forms utilize alternative start sites at Met 31 and Met165. Over aa 80-401, human CANT1 shares 89% aa sequence identity with mouse CANT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.