Calcineurin B Antibody - BSA Free

Western Blot: Calcineurin B Antibody [NBP1-32720] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:100000.

Immunohistochemistry-Paraffin: Calcineurin B Antibody [NBP1-32720] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.

Western Blot: Calcineurin B Antibody [NBP1-32720] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:1000.

Immunohistochemistry: Calcineurin B Antibody [NBP1-32720] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calcineurin B antibody (NBP1-32720) at 1:300 dilution.

Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (NBP1-32720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (NBP1-32720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ze, Bv, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calcineurin B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Calcineurin B Antibody - BSA Free (NBP1-32720) is a polyclonal antibody validated for use in IHC and WB. Anti-Calcineurin B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human Calcineurin B Recombinant protein.
• Predicted Species: Bovine (100%), Xenopus (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP3R1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:100000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Calcineurin B Antibody - BSA Free

• Calcineurin B
• calcineurin subunit B type 1
• CALNB1
• CNA2
• Cnb1
• MCIP1
• PP2B beta 1
• PPP3R1
• Protein phosphatase 2B regulatory subunit 1
• protein phosphatase 2B regulatory subunit B alpha
• protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I)
• protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I)
• protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
• Protein phosphatase 3 regulatory subunit B alpha isoform 1
• protein phosphatase 3, regulatory subunit B, alpha
• type I (19kDa)

Background

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Calcineurin B Antibody (NBP1-32720)(1)

Publication using NBP1-32720

• Filomena MC, Yamamoto DL, Carullo P Et al. Myopalladin knockout mice develop cardiac dilation and show a maladaptive response to mechanical pressure overload eLife 2021-09-24 [PMID: 34558411] (WB, Mouse)

Bioinformatics

• Gene Symbol: PPP3R1