Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free

Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).

Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).

• Reactivity: Hu
• Applications: IHC
• Clone: CALNA/2353
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07658).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human Calcineurin A protein (Uniprot: Q08209)
• Localization: Cytoplasmic and Cell Surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP3CA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free

• calcineurin A alpha
• Calcineurin A
• Calmodulin-dependent calcineurin A subunit alpha isoform
• CALN
• CALNA
• CALNAcatalytic subunit, alpha isoform
• CAM-PRP catalytic subunit
• CNA
• CNA1
• CNA1CALNA1
• EC 3.1.3.16
• PP2B
• PP3CA
• PPP2B
• PPP2BCCN1
• PPP3CA
• protein phosphatase 2B, catalytic subunit, alpha isoform
• protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
• protein phosphatase 3, catalytic subunit, alpha isozyme
• serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP3CA