Cadherin-6/KCAD Antibody (427909) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: Flow
• Clone: 427909
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Cadherin-6/KCAD Antibody (427909) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cadherin‑6/KCAD
  Ser54-Ala615
  Accession # P55285
• Specificity: Detects human Cadherin‑6/KCAD in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cadherin-8, -11, -12, -13, -17, rhECAD, rhMCAD, rhNCAD, rhRCAD, rhPCAD, or rhVECAD is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-6/KCAD Antibody (427909) [Alexa Fluor® 647]

• cadherin 6, type 2, K-cadherin (fetal kidney)
• cadherin, fetal kidney
• Cadherin6
• Cadherin-6
• CDH6
• KCAD
• K-cadherin (fetal kidney)
• K-Cadherin

Background

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium‑dependent, cell-to-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin‑6, also known as KCAD or K-cadherin, is a classical cadherin of 110‑120 kD that has at least one full length and two alternate splice forms ranging in size from 105‑120 kDa (3). Human Cadherin-6 is synthesized as a 790 amino acid (aa) type I transmembrane glycoprotein that contains a 18 aa signal peptide, a 35 aa prosequence, a 562 aa extracellular region, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (4, 5). There are five EC cadherin domains that are approximately 110 aa in length. This pattern is consistent with classical cadherin family molecules that are modular in their extracellular region and mediate calcium‑dependent cell-to-cell adhesion through their Ca⁺⁺-binding repeats (2). Due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat, Cadherin-6 can be further classified as a type II classical cadherin (4). One Cadherin‑6 splice variant (termed 6/2) shows a 9 aa substitution for the 94 aa that span residues 283 to 376 of the full-length extracellular region (3). A second splice variant shows a 36 aa substitution for the C-terminal 163 aa of the transmembrane and cytoplasmic region (6). The extracellular region of human Cadherin‑6 is 98% aa identical to rat Cadherin‑6 extracellular region, plus 60% and 58% aa identical to the extracellular regions of human cadherin 8 and 11, respectively. Cadherin-6 has high expression in kidney, brain, and cerebellum, and low expression in lung, pancreas, gastric mucosa, and cytotrophoblasts (4, 5, 7, 8, 9). Cadherin-6 is also found in renal, lung, and ovariancarcinoma (7, 10). As a classic cadherin, Cadherin-6 will form homodimers and promote intercellular adhesion with itself and possibly, cadherin-9 and -14 (4, 11).

1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
3. Mbalaviele, G. et al. (1998) J. Cell Biol. 141:1467.
4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
5. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
6. GenBank Accession # P55285.
7. Xiang Y.Y. et al. (1994) Cancer Res. 54:3034.
8. Marthiens V. et al. (2002) Mol. Cell Neurosci. 20:458.
9. MacCalman C.D. et al. (1998) Am J Reprod. Immunol. 39:96.
10. Sella, G.C. et al. (2001) Cancer Res. 61:6977.
11. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-6/KCAD Antibody (FAB2715R)(1)

Publication using FAB2715R

• Ruth Dannenfelser, Gregory M. Allen, Benjamin VanderSluis, Ashley K. Koegel, Sarah Levinson, Sierra R. Stark et al. Discriminatory Power of Combinatorial Antigen Recognition in Cancer T Cell Therapies Cell Systems 2020-09-10 [PMID: 32916097]

Bioinformatics

• Gene Symbol: CDH6
• Uniprot:
  • P55285()