Cadherin-4/R-Cadherin Antibody

Western Blot: Cadherin-4/R-Cadherin Antibody [NBP1-90370] - Differential expression of cadherin 13 and cadherin 4 proteins in 46BR.1G1 and 7A3 cells. Cell lysates from 46BR.1G1 and 7A3 cells were analyzed by Western blotting with anti-cadherin 13, anti-cadherin 4, and anti-alpha-tubulin antibodies. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0130561), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Cadherin-4/R-Cadherin Antibody [NBP1-90370] - Staining of human cell line U-251MG shows positivity in plasma membrane. Antibody staining is shown in green.

Western Blot: Cadherin-4/R-Cadherin Antibody [NBP1-90370] - Differential expression of cadherin 13 and cadherin 4 proteins in 46BR.1G1 and 31W cells. Cell lysates from 46BR.1G1 and 31W cells were analyzed by Western blotting with antibodies against the indicated proteins. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0130561), licensed under a CC-BY license.

Product Discontinued

Cadherin-4/R-Cadherin Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YLIDINDNAPELLPKEAQICERPNLNAINITAADADVDPNIGPYVFELPFVPAAVRKNWTITRLNGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPLSNTSIIKVKVCPCD
• Predicted Species: Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDH4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cadherin-4/R-Cadherin Antibody

• CAD4
• cadherin 4, type 1, preproprotein
• cadherin 4, type 1, R-cadherin (retinal)
• Cadherin4
• Cadherin-4
• CDH4
• FLJ22202
• FLJ40547
• MGC126700
• MGC138355
• RCAD
• R-CAD
• R-Cadherin
• Retinal cadherin

Background

CDH4 - cadherin 4, type 1, R-cadherin (retinal)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-4/R-Cadherin Antibody (NBP1-90370)(2)

Publications using NBP1-90370

• Cremaschi P, Oliverio M, Leva V et al. Chronic Replication Problems Impact Cell Morphology and Adhesion of DNA Ligase I Defective Cells PLoS ONE. 2015-07-08 [PMID: 26151554] (WB, Human)
  
  Details:
  Cadherin-4/R-Cadherin antibody was used at 1:300 dilution for WB assay on lysates from Human SV40-transformed 46BR.1G1 fibroblasts, 7A3 cells and 31W cells (Figure 5 and 6).
• Aschauer L, Gruber LN, Pfaller W et al. Delineation of the Key Aspects in the Regulation of Epithelial Monolayer Formation. Mol Cell Biol 2013-07-01 [PMID: 23608536]

Bioinformatics

• Gene Symbol: CDH4