CACNG5 Antibody

Western Blot: CACNG5 Antibody [NBP2-76921] - Western blot analysis of CACNG5 on LOVO cell lysate using anti-CACNG5 antibody at 1/200 dilution.

Immunocytochemistry/Immunofluorescence: CACNG5 Antibody [NBP2-76921] - ICC staining CACNG5 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: CACNG5 Antibody [NBP2-76921] - Flow cytometric analysis of PANC-1 cells with CACNG5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CACNG5 Antibody [NBP2-76921] - ICC staining CACNG5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CACNG5 Antibody [NBP2-76921] - ICC staining CACNG5 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

CACNG5 Antibody Summary

• Immunogen: Synthetic peptide within Human CACNG5 aa 68-117 / 275. (SwissProt: Q9UF02 Human; SwissProt: Q8VHW8 Rat)
• Localization: Plasma membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CACNG5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACNG5 Antibody

• calcium channel, voltage-dependent, gamma subunit 5
• MGC126656
• MGC126682
• Neuronal voltage-gated calcium channel gamma-5 subunit
• voltage-dependent calcium channel gamma-5 subunit

Background

FUNCTION: Thought to stabilize the calcium channel in an inactivated (closed) state.; Subcellular location: Membrane; Single-pass type I membrane protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNG5