CACNG4 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

CACNG4 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CACNG4.

  Source: E. coli

  Amino Acid Sequence: TLSREPLKVTTAASYSPDQEASFLQVHDFFQQDLKEGFHVSMLNRRTTPV

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: CACNG4
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-55863.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for CACNG4 Recombinant Protein Antigen

• calcium channel, voltage-dependent, gamma subunit 4
• MGC11138
• MGC24983
• neuronal voltage-gated calcium channel gamma-4 subunit
• TARP gamma-4
• transmembrane AMPAR regulatory protein gamma-4
• voltage-dependent calcium channel gamma-4 subunit

Background

The CACNG4 gene encodes a 327 amino acid long, 36 kDA type I transmembrane AMPA receptor regulatory protein (TARP). The function of this protein is to regulate circulation and channel gating of the AMPA receptors by monitoring rates of activation and deactivation, as well as regulating gate desensitization and resensitization but is not subunit-specific. The CACNG4 gene interacts with CACNG2, CACNG3, CACNG7, AKAP5, and CAMK2D genes in CREB transcription pathways, trafficking of AMPA receptors, MAPK signaling pathways, and cardiac muscle contractions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CACNG4