CACNG4 Antibody

Western Blot: CACNG4 Antibody [NBP2-76920] - Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: CACNG4 Antibody [NBP2-76920] - ICC staining Cacng4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CACNG4 Antibody [NBP2-76920] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.

Flow Cytometry: CACNG4 Antibody [NBP2-76920] - Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CACNG4 Antibody [NBP2-76920] - ICC staining Cacng4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CACNG4 Antibody [NBP2-76920] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.

Product Discontinued

CACNG4 Antibody Summary

• Immunogen: Synthetic peptide within Human CACNG4 aa 189-238 / 327. (SwissProt: Q9UBN1 Human; SwissProt: Q8VHW9 Rat)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CACNG4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACNG4 Antibody

• calcium channel, voltage-dependent, gamma subunit 4
• MGC11138
• MGC24983
• neuronal voltage-gated calcium channel gamma-4 subunit
• TARP gamma-4
• transmembrane AMPAR regulatory protein gamma-4
• voltage-dependent calcium channel gamma-4 subunit

Background

The CACNG4 gene encodes a 327 amino acid long, 36 kDA type I transmembrane AMPA receptor regulatory protein (TARP). The function of this protein is to regulate circulation and channel gating of the AMPA receptors by monitoring rates of activation and deactivation, as well as regulating gate desensitization and resensitization but is not subunit-specific. The CACNG4 gene interacts with CACNG2, CACNG3, CACNG7, AKAP5, and CAMK2D genes in CREB transcription pathways, trafficking of AMPA receptors, MAPK signaling pathways, and cardiac muscle contractions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNG4