CACNB4 Antibody

Western Blot: CACNB4 Antibody [NB100-1485] - Staining of human bone marrow lysate (35 ug protein in RIPA buffer). Antibody at 0.2 ug/mL. Detected by chemiluminescence.

Product Discontinued

CACNB4 Antibody Summary

• Immunogen: Peptide with sequence CSPGGYSHDSRHRL corresponding to C-Terminus according to NP_000717.2, NP_001005746.1.
• Specificity: This antibody is expected to recognise all reported protein isoforms of human CACNB4 (NP_001005747.1; NP_000717.2; NP_001005746.1; )
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CACNB4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 60+55 kDa band bands observed in human bone marrow lysates and in transfected HEK293 transiently expressing CACNB4 (calculated MW of 58.2 kDa band according to NP_000717.2 and 54.7 kDa band according to NP_001005747.1).
• Control:

  CACNB4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACNB4 Antibody

• CAB4
• CACNB4
• CACNLB4
• calcium channel beta-4 subunit
• calcium channel, voltage-dependent, beta 4 subunit
• EJM
• EJM4
• EJM6
• voltage-dependent L-type calcium channel subunit beta-4

Background

This gene encodes a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CACNB4 Antibody (NB100-1485)(1)

Publication using NB100-1485

• Badou A, Basavappa S, Desai R et al. Requirement of voltage-gated calcium channel beta4 subunit for T lymphocyte functions. Science 2005-01-07 [PMID: 15637280]

Bioinformatics

• Gene Symbol: CACNB4
• Entrez:
  • 785(Human)
  • 12298(Mouse)
  • 58942(Rat)
• Uniprot:
  • NP_000717(Human)