CACNB4 Antibody (S10-7)

Western Blot: CACNB4 Antibody (S10-7) [NBP2-12908] - analysis of hamster T-CHO cell lysate showing detection of CACNB4 Calcium Channel protein using Mouse Anti-CACNB4 Calcium Channel Monoclonal Antibody, Clone S10-7 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-CACNB4 Calcium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Discontinued

CACNB4 Antibody (S10-7) Summary

• Immunogen: Synthetic peptide amino acids 458-474 of rat CavB4
• Localization: Cytoplasm , Cell Membrane , Synapse
• Specificity: Detects approx 50kDa. No cross-reactivity against rat Cab Beta1b, Cav beta2a, and Cav betas3 in transfected cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CACNB4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of Cav beta 4 Antibody was sufficient for detection of CavB4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CACNB4 Antibody (S10-7)

• CAB4
• CACNB4
• CACNLB4
• calcium channel beta-4 subunit
• calcium channel, voltage-dependent, beta 4 subunit
• EJM
• EJM4
• EJM6
• voltage-dependent L-type calcium channel subunit beta-4

Background

1 ug/mL was sufficient for detection of Cav beta4 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNB4
• Uniprot:
  • NP_000717.2(Human)