CACNA1E Antibody

Immunocytochemistry/Immunofluorescence: CACNA1E Antibody [NBP2-76923] - ICC staining CaV2.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: CACNA1E Antibody [NBP2-76923] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CaV2.3 antibody. Counter stained with hematoxylin.

Flow Cytometry: CACNA1E Antibody [NBP2-76923] - Flow cytometric analysis of 293T cells with CaV2.3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: CACNA1E Antibody [NBP2-76923] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaV2.3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: CACNA1E Antibody [NBP2-76923] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CaV2.3 antibody. Counter stained with hematoxylin.

Product Discontinued

CACNA1E Antibody Summary

• Immunogen: Synthetic peptide within rat CACNA1E aa 875-924 / 2,222. (SwissProt: Q15878 Human; SwissProt: Q61290 Mouse; SwissProt: Q07652 Rat)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CACNA1E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 262 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACNA1E Antibody

• BII
• Brain calcium channel II
• CACH6
• CACH6calcium channel, R type, alpha-1 polypeptide
• CACNA1E
• CACNL1A6
• CACNL1A6calcium channel, voltage-dependent, alpha 1E subunit
• calcium channel, voltage-dependent, R type, alpha 1E subunit
• Cav2.3
• L type, alpha-1 polypeptide
• L type, alpha-1 polypeptide, isoform 6
• voltage-dependent calcium channel alpha 1E subunit
• voltage-dependent R-type calcium channel subunit alpha-1E
• voltage-gated calcium channel alpha 1E subunit
• Voltage-gated calcium channel subunit alpha Cav2.3

Background

Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels contaning alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.; Subcellular location: Membrane; Multi-pass membrane protein.; Tissue specificity: Expressed in neuronal tissues and in kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNA1E