c-jun Antibody (E254)

Product Discontinued

c-jun Antibody (E254) Summary

• Immunogen: A synthetic peptide corresponding to N-terminal residues of human c-Jun was used as immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: JUN
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 1:40
  • Western Blot 1:2000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 43kDa. Flow Cytometry was reported in scientific literature.
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  c-jun Overexpression Lysate

Reactivity Notes

Predicted to cross-react with Pig, based on sequence identity.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for c-jun Antibody (E254)

• Activator protein 1
• AP1
• AP-1
• cJun
• c-Jun
• enhancer-binding protein AP1
• Jun activation domain binding protein
• jun oncogene
• jun proto-oncogene
• JUN
• Proto-oncogene c-Jun
• transcription factor AP-1
• V-jun avian sarcoma virus 17 oncogene homologp39
• v-jun sarcoma virus 17 oncogene homolog (avian)
• v-jun sarcoma virus 17 oncogene homolog

Background

c-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for c-jun Antibody (NB110-55569)(3)

Publications using NB110-55569

• Martin KK, Parvin S, Garraway SM. Peripheral inflammation accelerates the onset of mechanical hypersensitivity after spinal cord injury and engages TNFa signaling mechanisms J. Neurotrauma 2018-12-06 [PMID: 30520675] (WB, Rat)
• Garraway SM, Woller SA, Huie JR et al. Peripheral noxious stimulation reduces withdrawal threshold to mechanical stimuli after spinal cord injury: role of tumor necrosis factor alpha and apoptosis. Pain 2014-11-01 [PMID: 25180012]
• Huen NY, Pang AL, Tucker JA et al. Up-regulation of proliferative and migratory genes in regulatory T cells from patients with metastatic castration-resistant prostate cancer. Int J Cancer 2013-01-15 [PMID: 23319273] (FLOW, Human)

Review for c-jun Antibody (NB110-55569) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC Mouse 12/28/2011

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse
• Species: Mouse

Bioinformatics

• Gene Symbol: JUN
• Entrez:
  • 3725(Human)
  • 16476(Mouse)
  • 24516(Rat)
• Uniprot:
  • P05412()