BTAF1 Antibody - BSA Free

Immunoprecipitation: BTAF1 Antibody [NB100-57502] - Detection of Human BTAF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20 percent of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody NB100-57502 used for IP at 3 mcg/mg lysate (A and B). BTAF1 was also immunoprecipitated by rabbit anti-BTAF1 antibodies NB100-57501 and NB100-57503, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 30 seconds (B).

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BTAF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BTAF1 Antibody - BSA Free (NB100-57502) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1050 and 1100 of human RNA polymerase II, B-TFIID transcription factor-associated, 170kDa using the numbering given in entry NP_003963.1 (GeneID 9044).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BTAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BTAF1 Antibody - BSA Free

• ATP-dependent helicase BTAF1
• BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1homolog, S. cerevisiae)
• B-TFIID transcription factor-associated 170 kDa subunit
• EC 3.6.1
• EC 3.6.4.-
• MOT1MGC138406
• TAF(II)170KIAA0940
• TAF-172
• TAF172BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170 kD (Mot1homolog, S. cerevisiae)
• TAFII170TATA-binding protein-associated factor 172
• TBP-associated factor 172

Background

BTAF1 is a member of the SNF2-like family of ATPases that associates with the TATA-binding protein (TBP) and TAFII170 (TAF172) to form the B-TF-IID complex. As part of the general transcription machinery, BTAF1 acts as a regulator of TBP and RNA polymerase II transcription. Alternate names for BTAF1 include BTAF-1, RNA polymerase II, B-TFIID transcription factor-associated, 170kDa, TAF172, TBP-associated factor 172, TAFII170, B-TFIID transcription factor-associated, 170kDa, and MOT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTAF1
• Entrez:
  • 9044(Human)
• Uniprot:
  • NP_003963.1(Human)