BRF1 Antibody

Images

 
Western Blot: BRF1 Antibody [H00002972-B01P] - Analysis of BRF1 expression in transfected 293T cell line by BRF1 polyclonal antibody. Lane 1: BRF1 transfected lysate(52.03 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: BRF1 Antibody [H00002972-B01P] - Analysis of purified antibody to BRF1 on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

BRF1 Antibody Summary

Immunogen
BRF1 (NP_663718.1, 1 a.a. - 473 a.a.) full-length human protein. MTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQLEQVLSKKLEEVEGEISSYQDAIEIELENSRPKAKGGLASLAKDGSTEDTASSLCGEEDTEDEELEAAASHLNKDLYRELLGGAPGSSEAAGSPEWGGRPPALGSLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAIEKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSASARKLSRRRTPASRSGADPVTSVGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARPQAVLVESGPVSYHADEEADEEEPDEEDGEPCVSALQMMGSNDYGCDGDEDDGY
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
BRF1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
It has been used for WB and IF.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BRF1 Antibody

  • B-related factor 1
  • BRF1 homolog, subunit of RNA polymerase III transcription initiation factorIIIB (S. cerevisiae)
  • BRF-1
  • BRFFLJ42674
  • general transcription factor IIIB, 90kD subunit
  • GTF3BTAFIII90
  • hBRFMGC105048
  • hTFIIIB90
  • TAF3B2
  • TAF3CB - related factor 1
  • TATA box binding protein (TBP)-associated factor 3C
  • TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3Bsubunit 2
  • TATA box binding protein (TBP)-associated factor, RNA polymerase III, subunit 2
  • TATA box-binding protein-associated factor, RNA polymerase III, subunit 2
  • TBP - associated factor, RNA polymerase III, 90kD
  • TF3B90
  • TFIIIB90FLJ43034
  • transcription factor IIIB 90 kDa subunit

Background

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Two alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. Other transcript variants are possible, but their full-length natures have not been completely characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BRF1
Uniprot