Bora Antibody (694730)

Bora was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Bora Monoclonal Antibody (Catalog # MAB6856) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 694730
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Bora Antibody (694730) Summary

• Immunogen: E. coli-derived recombinant human Bora
  Gly2-Ala180
  Accession # Q6PGQ7
• Specificity: Detects human Bora in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BORA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bora Antibody (694730)

• Aurora Borealis
• Bora
• C13orf34
• chromosome 13 open reading frame 34
• FLJ22624
• HsBora
• protein aurora borealis

Background

Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins.  It is ubiquitously expressed, and plays a key role in cell cycle progression.  Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis.  Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210.  Bora, Aurora-A and Plk1 all appear to form a complex during G2.  Bora predisposes Plk1 to the actions of Aurora-A.  Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora.  This initiates BORA dissociation from Aurora-A with subsequent ubiquitination and degradation.  Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites.  Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa.  There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87.  Over aa 2-180, human Bora shares 84% aa identity with mouse Bora.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BORA
• Uniprot:
  • Q6PGQ7(Human)