BOP1 Antibody (T7-P1B7*A8) - BSA Free

Western Blot: BOP1 Antibody (T7-P1B7-A8) [NBP2-50124] - Analysis of BOP1 in transfected Jurkat cell lysate.

Immunohistochemistry-Paraffin: BOP1 Antibody (T7-P1B7-A8) [NBP2-50124] - Analysis of BOP1 in a paraffin embedded human breast cancer cross section.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: T7-P1B7*A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BOP1 Antibody (T7-P1B7*A8) - BSA Free Summary

• Immunogen: Peptide Sequence - KGHVLTRDLG
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BOP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1/3
  • Immunohistochemistry-Paraffin 1/3
  • Western Blot 1:100 - 1:2000
• Application Notes: Antigen retrieval for IHC: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Positive control(s): ELISA - peptide immunogen; WB - Jurkat whole cell lysate; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BOP1 Antibody (T7-P1B7*A8) - BSA Free

• block of proliferation 1
• KIAA0124Block of proliferation 1 protein
• ribosome biogenesis protein BOP1

Background

BOP1 (block of proliferation 1) is a component of the PeBoW (Pes1, Bop, WDR12) complex that plays a role in the processing of 28S and 5.8S ribosomal RNAs and the formation of the 60S ribosome. BOP1 contains seven WD repeats and was originally identified as a cDNA which inhibited cell proliferation. The deregulation of the BOP1 gene is proposed to contribute to colorectal tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BOP1