Rat BNP-45 ELISA Kit (Colorimetric)

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ELISA: NPPB ELISA Kit [Biotin] [KA0979] - Standard curve

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Cell Culture Samples, Plasma, Serum and Tissue Extract
Standard Curve Range
0.031 to 1 ng/mL
Sensitivity
12 pg/mL

Order Details

Rat BNP-45 ELISA Kit (Colorimetric) Summary

Description
Nppb (Rat) ELISA Kit is designed for detection of rat Nppb in rat plasma, serum, tissue extract, and cell culture supernatants.
Specificity
This Rat BNP-45 ELISA Kit (Colorimetric) is specific to Brain Natriuretic Peptide 45 (aa 77-121).
Standard Curve Range
0.031 to 1 ng/mL
Sensitivity
12 pg/mL
Inter-Assay
CV% < 7.80%
Intra-Assay
CV% < 4.20%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
NPPB

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publication using KA0979.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Rat BNP-45 Antibody (50x): A 50-fold biotinylated polyclonal antibodyagainst rat BNP-45.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. MIX Diluent Concentrate (10x): A 10-fold buffered protein base.
  4. Rat BNP-45 Microplate: A 96 well polystyrene microplate coated with a polyclonalantibody against rat BNP-45.
  5. Rat BNP45 Standard: Rat BNP-45 in a buffered protein base (lyophilized).
  6. Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of theindividual assay.
  7. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction
  8. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat BNP-45 ELISA Kit (Colorimetric)

  • BNP
  • brain type natriuretic peptide
  • Gamma-brain natriuretic peptide
  • natriuretic peptide precursor B
  • natriuretic peptides B
  • natriuretic protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for BNP-45 ELISA Kit (KA0979)(1)

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Product General Protocols

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FAQs for BNP-45 ELISA Kit (KA0979). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol NPPB