BMPR-II Antibody

Western Blot: BMPR-II Antibody [NBP1-32218] - Wild-type (WT) and BMPR2 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BMPR2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: BMPR-II Antibody [NBP1-32218] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BMPR2 antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Rabbit Polyclonal BMPR-II Antibody [NBP1-32218] - Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. BMP-2, NBP1-32218, was used at a dilution of 1:1000 and RUNX/CBFA1, NBP2-24755 was used at a dilution of 1:1000 in TBS Tween and 5% powdered milk. The protein was labeled at 150 kDa. Quantification of BMP-2 in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in the iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to the tibial bone defect, G2: cellularized graft for 6 weeks applied to the tibial bone defect, G3: cellularized graft for 8 weeks applied to the tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation. Image from a verified customer review.

• Reactivity: Hu, Gt, Mu, Rt, Po, Bv, RM, Sh
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

BMPR-II Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human BMPR-II. The exact sequence is proprietary.
• Localization: Membrane
• Predicted Species: Mouse (92%), Rat (92%), Porcine (92%), Bovine (93%), Rhesus Macaque (98%), Sheep (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BMPR2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (86%). Goat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for BMPR-II Antibody

• BMP type II receptor
• BMP type-2 receptor
• BMPR2
• BMPR-2
• BMPR3
• BMPRII
• BMPR-II
• BMPR-IIprimary pulmonary hypertension 1
• Bone morphogenetic protein receptor type II
• bone morphogenetic protein receptor type-2
• bone morphogenetic protein receptor, type II (serine/threonine kinase)
• BRK-3
• EC 2.7.11
• EC 2.7.11.30
• FLJ41585
• FLJ76945
• PPH1BMR2
• T-ALK
• type II activin receptor-like kinase
• type II receptor for bone morphogenetic protein-4

Background

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for BMPR-II Antibody (NBP1-32218) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Goat.

reviewed by: Inácio Viana WB Goat 05/13/2025

Summary

• Application: Western Blot
• Sample Tested: Bone Extracts,Bone
• Species: Goat
• Lot: 43118 21/12/2024

Comments

• Comments: ***Bio-Techne Response: This review reflects a new species or application tested on a primary antibody.***
  Quantification of BMP-2 in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in the iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to the tibial bone defect, G2: cellularized graft for 6 weeks applied to the tibial bone defect, G3: cellularized graft for 8 weeks applied to the tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation.

Bioinformatics

• Gene Symbol: BMPR2